JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ007724.1
Database:	nt	Go to original record
Description:	Orobanche minor chloroplast trnL(UAA) gene containing group I intron
Taxon:	Orobanche minor
Original size:	986 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	189	729	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct ttgtatggaa
51    acttgctaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctatttta tcaaaacaaa gtttagaaaa
151   agaaaagggg ataggtgcag agactcaatg gaagttgttc taacaaacag
201   agttgattgc gttggtagag aaatctttcc attgtaactt cagaaaggat
251   gaaggataaa ctgattaatg atgacccgaa cctatatctt ttttttgaat
301   attaaaaaga attggtgcga atttcttcta tattgaagaa agaatgaata
351   ttcatttatc aaatcattca ctccatagtt tgataaatct tttttaaaaa
401   gaactgatta atcagacgag aataaagata gagtcccatt ctacatgtca
451   atacatgtca atactggcaa caattaaatt tatagtaaga ggaaaatccg
501   tcgattttaa aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details