JRC GMO-Amplicons | EURL GMFF

Record ID:	OU343025.1
Database:	nt	Go to original record
Description:	Patellifolia patellaris genome assembly, organelle: plastid:chloroplast
Taxon:	Patellifolia patellaris
Original size:	151798 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46929	47540	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt tttcaaaagc aaaaaaagaa
151   ggattccgaa aacaagaata aaaaaaggat aggtgcagag actcaaagga
201   agctgttcta acaaatgggg ttgactgtgc tgttctaaga attcttctgc
251   cgaaactcca gtccaaaatg ggtgaagaat atactatatc aaatgattaa
301   tgacaccccg aatctattct gtattttttt tctaaatttt tagatattta
351   gaatatgaaa tgaaaaatat atatgaatag gaaaagatgg aagaattctt
401   gtgaatcgat ttcaagttga aaaaagactc aaatattcat tcatcaaatc
451   attcactcca taatctgata gatctttgga agaactgatt aatcgtacga
501   gaataaagat agagtcccat tctacatgtc aatactgaca acaatgaaat
551   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details