JRC GMO-Amplicons | EURL GMFF

Record ID:	KF488310.1
Database:	nt	Go to original record
Description:	Rytigynia sp. Verstraete & Lemaire 371 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Rytigynia sp. Verstraete & Lemaire 371
Original size:	1672 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	795	1352	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaac cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaaatcaaat gattaatgac
301   gcctcgactg aatccgtatt ttttatatga aaacggaaga attggtgtga
351   atagattcca cattgaagaa agaatcgaat attcattgat taaatgattc
401   actccatagt ctgatagatc ttttcaagaa ttgattaatc ggacgagaat
451   aaagatagag tcccgttcta catgtcaatg tcggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details