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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AB863184.1
Database: nt Go to original record
Description: Cauliflower mosaic virus DNA, complete genome, isolate: TUR249.
Taxon: Cauliflower mosaic virus
Original size: 8023 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 7165 7371 + 207 bp
Amplicon: 207 bp 
1     gcgcctacca atgccatcaa tgcgataaag gaaaggctat cgttgaagat
51    aagcctgccg acagtggtcc agcagacagc tcgggtccac ccaccacgaa
101   agaaaacgtg gaaaaagaag atgccgtttc cactacgaca tcttcaaagc
151   aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
201   cactatc

View other 13 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerB 100.0 20 bp 0 0 207 188 9.87e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.