JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ751064.1
Database:	nt	Go to original record
Description:	Lilium concolor var. pulchellum chloroplast, complete genome.
Taxon:	Lilium concolor var. pulchellum
Original size:	152626 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45100	45715	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttttaa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagttgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   attcttctat caaaattaca gagaggatgc ccgccctata tacgtatata
301   tacatactga ccatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatatatat atgaaaaatt taagagttat tgtaaatcta
401   ttccatattc caatcaaagt ttaaggaaga atcgaatatt cagtgatcaa
451   attattcatt ccagagtctg tatagatctt tttaaaaact gattattcgg
501   acgagaataa agagagagtc ccattctacg tgtcaatatc gacaacaatg
551   aaatttatag taaaaggaaa atccgtcgac tttataagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details