Match details
Record ID:
MW026678.1
Database:
nt
Go to original record
Description:
Plant expression vector pZG66, complete sequence.
Taxon:
Plant expression vector pZG66
Original size:
13025 bp
View other 21 matches for this record
Method:
QL-CON-00-015
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
Target: CaMV P-35S-bar
View other 358 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
1
11964
12096
-
133 bp
1 ctatccttcg caagaccttc ctctatataa ggaagttcat ttcatttgga
51 gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga
101 gaccatgagc ccagaacgac gcccggccga cat
View other 2 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
95.2
21 bp
0
1
2
21
3.34e-07
primerB
100.0
22 bp
0
0
133
112
5.51e-10
probe
100.0
25 bp
0
0
22
46
1.41e-11
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.