JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ274163.1
Database:	nt	Go to original record
Description:	Leonurus cardiaca chloroplast, complete genome.
Taxon:	Leonurus cardiaca
Original size:	151831 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46508	47070	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taagaaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga tgaaggagaa acgtatctat cgaatactat atcaaatgat
301   taatgatggc ccgaatccgt attttttaat atgaaaaata gaagaattgg
351   tgggaattga ttctatattg aagaaaaaag agaatattca ttcatcaaat
401   cattcactcc acagtccgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caatactggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

View other 20 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details