JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ397802.1
Database:	nt	Go to original record
Description:	Amaranthus dubius chloroplast, complete genome.
Taxon:	Amaranthus dubius
Original size:	150520 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47405	47998	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaaaaata
151   cggattcaga aagcaagaat aaaaaaaagg ataggtgcag agactcaaag
201   gaagctgttc taacaaatgg agttgattgt gctgggttgt tcgaaattaa
251   tgacacttcg aatctattct gtatttctat ttttagaatt tttagatatt
301   tcttatttct agataatata gaatatgaaa ttttctagaa ataaaaatga
351   atatgaaaaa atggaagaat ttttgtgact ctatttcaag ttgaaaaaag
401   aatcaaatat tcatcaaatc attcactcca tagtctgata gatctttctt
451   tgaaagaacc ggttaatcgg ttactcggac gagaataaag atagagtccc
501   attatacatg tcaatactga caacaatgaa atttatagta cgaggaaaat
551   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details