JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ555773.1
Database:	nt	Go to original record
Description:	Thunbergia erecta chloroplast, complete genome.
Taxon:	Thunbergia erecta
Original size:	152202 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47483	48015	+	533 bp

Amplicon:

533 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt atcaaaaaaa aggttcagaa
151   aacggatagg tgcagagact caatggaagc tattctaaca aatggagttg
201   actgcgttgg taaagtaaag gaatctttcc atcgaaactt cagaaaggat
251   gaaggctaaa cgtatctatt gaatactggc cctaatctgt atttttgtac
301   atgaaaaatg gaagaattag tgtgaattga ttccacattg aagaaagaat
351   cgaatattca ttcatcaaat cattcactcc atagtacgat agatctttta
401   aagaactgat taatcggacg agaataaaga tagagtccca ttctacatgt
451   caataccggc aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
501   caaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	533	514	2.40e-08

Match details