JRC GMO-Amplicons | EURL GMFF

Record ID:	KF905189.1
Database:	nt	Go to original record
Description:	Elymus excelsus isolate EI_201203 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Campeiostachys dahurica var. excelsa
Original size:	1006 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagt ggtcctcgaa
151   ctagaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatcaagt
201   taattacgtt gtgttggtag tggaattcct tctaaattag agggagaagg
251   gctttataca tctaataaac acgtatagat actggcatag caaacgatta
301   atcacagaac ccatatcata atataggttc tttattcttt tttagaatga
351   aattaggaaa gattatgaaa taaaaaattc agaatttttt tgaattattg
401   tgaattcatt ccaatcgaat attgagtaat caaatccttc aattcatagt
451   tttcgagatc ttttaaaaag tggattaatc ggacgaggac aaagagagag
501   tcccattcta catgccaata ctggcaacaa tgaaatttct agtaaaagga
551   aaatccgtcg actttataag ttgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details