JRC GMO-Amplicons | EURL GMFF

Record ID:	KF922718.1
Database:	nt	Go to original record
Description:	Neobartsia inaequalis chloroplast, partial genome.
Taxon:	Neobartsia inaequalis
Original size:	151349 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47350	47900	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa agattcagaa
151   aacgaaaaaa aggataggtg cagagactct atggaagttg ttctaacaaa
201   tggggttgat tacgctggta gaggaatgaa acttcagaaa ggctgaagga
251   taaaggcatc tattgactac tatatcaata gattgagtaa tgatggccca
301   aatctgtatc tgtatttttg aatacgcaaa atggaagaat tggtgtgaat
351   tgattccata ttgaaaaaag aagattcatt gatcaaataa ttcactccat
401   agtccgatag atcgtttaaa gaactgatta atcggacgag aataaagata
451   gagtcccatt ctacatgtca ataccggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details