Match details

Record ID: KF537344.1
Database: nt Go to original record
Description: Cloning vector pBSVirHygGW, complete sequence.
Taxon: Cloning vector pBSVirHygGW
Original size: 10770 bp

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Method: QL-ELE-00-008 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase promoter.
Target:P-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1868 1961 - 94 bp

Amplicon: 94 bp
1     aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
51    cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctga 

View other 3895 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 3.03e-12
primerB 100.0 24 bp 0 0 94 71 3.54e-11
probe 100.0 26 bp 0 0 28 53 3.03e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.