JRC GMO-Amplicons | EURL GMFF

Record ID:	KC429716.1
Database:	nt	Go to original record
Description:	Lonicera chrysantha tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lonicera chrysantha
Original size:	1002 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	423	1002	-	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaccccgga ataataaaaa
101   tgggcaatga gccaaccagt tttccgaaaa caagggttta gaaagcaaaa
151   atcaaaaagg ataggtgcag agactcaatg gaagctgttc taacaaacgg
201   agttgactgt cgtgtgttgg tagaaagaat ccttccatag aaacttcaga
251   aaggataaac ctataaacat agatatacgt attgaaatgc tatgatacta
301   tatcaaatga ttaatgacga tcctaatttg tatctgtatt ttatatatat
351   caaaatggaa gaattgttgt gaagtgattc catattgaag aaagaatcaa
401   atattcattg atcaaatcat tcactccata gtctgatcga tcttttgaag
451   aactgattaa tcggacgaga ataaagatag agtcccattc tacatgtcaa
501   gaccggcaac aatgaaattt atagtaagag gaaaatccgt cgactttaga
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details