JRC GMO-Amplicons | EURL GMFF

Record ID:	KC429715.1
Database:	nt	Go to original record
Description:	Lonicera xylosteum tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lonicera xylosteum
Original size:	1004 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	423	1004	-	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gatgaccttg gtatgaaacc
51    taccaagtga gactttcaaa tcagagaaac cccggaatta ataaaaatgg
101   gcaatcctga gccaaatcca gttttccgaa aacaagggtt tagaaagcaa
151   aaatcaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaac
201   ggagttgact gtcgtgtgtt ggtagaaaga atccttccat agaaacttca
251   gaaaggataa acctataaac atagatatac gtattgaaat gctatgatac
301   tatatcaaat gattaatgac gatccgaatt tgtatctgta ttttatatat
351   atcaaaatgg aagaattgtt gtgaagtgat tccatattga agaaagaatc
401   aaatattcat tgatcaaatc attcactcca tagtctgatc gatcttttga
451   agaactgatt aatcggacga gaataaagat agagtcccat tctacatgtc
501   aagaccggca acaatgaaat ttatagtaag aggaaaatcc gtcgacttta
551   gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details