JRC GMO-Amplicons | EURL GMFF

Record ID:	KC429714.1
Database:	nt	Go to original record
Description:	Lonicera venulosa tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lonicera venulosa
Original size:	1005 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	430	1005	-	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgggaagttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atccagtttt ccgaaaacaa gggtttagaa
151   agcaaaaatc aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaacggagt tgactgtcgt gtgttggtag aaagaatcct tccatagaaa
251   cttcagaaag gataaaccta taaacataga tatacgtatt gaaatgctat
301   gattgattaa tacgatccta aatttgtatc tgtattttat atatatcaaa
351   tggaagaaat tgttgtgaag tgattccata ttgaagaaag aatcaaatat
401   tcattgatca aatcattcac tccatagtct gatcgatctt ttgaagaact
451   gattaatcgg acgagaataa agatagagtc ccattctaca tgtcaagacc
501   ggcaacaatg aaatttatag taagaggaaa atccgtcgac tttagaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details