JRC GMO-Amplicons | EURL GMFF

Record ID:	KC429712.1
Database:	nt	Go to original record
Description:	Lonicera caerulea var. emphyllocalyx tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lonicera caerulea var. emphyllocalyx
Original size:	1001 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	430	1001	-	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gtaattgtgg gactaagtga
51    gaactttctt cgggaaaccc cggaattaat aaaaatgggc aatcctgagc
101   caaatccagt tttccgaaaa caagggttta gaaagcaaaa atcaaaaagg
151   ataggtggca gaggactcaa tggaagctgt tctaacaaac ggagttgact
201   gtcgtgtgtt ggtagaaaga atccttccat agaaacttca gaaaggataa
251   acctataaac atagatatac gtattgaaat gctatgatac tatatcaaat
301   gattaatgac gatcctaatt tgtatctgta ttttatatat atcaaaatgg
351   aagaattgtt gtgaagtgat tccatattga agaaagaatc aaatattcat
401   tgatcaaatc attcactcca tagtctgatc gatcttttga agaactgatt
451   aatcggacga gaataaagat agagtcccat tctacatgtc aagaccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta gaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details