JRC GMO-Amplicons | EURL GMFF

Record ID:	KC429708.1
Database:	nt	Go to original record
Description:	Lonicera caerulea var. pallasii tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lonicera caerulea var. pallasii
Original size:	1015 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	429	1015	-	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atccagtttt ccgaaaacaa gggtttagaa
151   agcaaaaatc aaaaaggaca ggtgcagaga ctcaatggaa gctgttctaa
201   caaacggagt tgactgtcgt gtgttggtag aaagaatcct tccatagaaa
251   cttcagaaag gataaaccta taaacataga tatacgtatt gaaatgctat
301   gatactatat caaatgatta atgacgatcc taatttgtat ctgtatttta
351   tatatatcaa aatggaagaa ttgttgtgaa gtgattccat attgaagaaa
401   gaatcaaata ttcattgatc aaatcattca ctccatagtc tgatcgatct
451   tttgaagaac tgattaatcg gacgagaata aagatagagt cccattctac
501   atgtcaagac cggcaacaat gaaatttata gtaagaggaa aatccgtcga
551   ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details