JRC GMO-Amplicons | EURL GMFF

Record ID:	AM397157.1
Database:	nt	Go to original record
Description:	Podophyllum peltatum tRNA-Leu gene (partial), trnL-trnF intergenic spacer and tRNA-Phe gene (partial), specimen voucher T. Borsch 3393 (BONN)
Taxon:	Podophyllum peltatum
Original size:	951 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	540	+	540 bp

Amplicon:

540 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactgag tgataacttt caaattyaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcgg aaaagggata ggtgcagaga
151   ctcgatggaa gctgttctaa cgaatgaagt tggtcgaaga aaatctttct
201   attaaaactc cagaaaggat gaacctatat atgatatata ggtatacata
251   ctgaaataaa atagaaaaga ttaatgaaat acgaatcctt tatttaactt
301   atacaaaaat tgaaaaattt ttccgaatcg attccatgtg gaagtaagga
351   tcgaatattc actgatcaaa tcagccactt tagagtatag tatgatatag
401   attttgtaag aactaactaa ttggacgaga ataaagatag agtcccattc
451   tacatgtcaa taccgacaac aatgaaattt atagtaagag gaaaatccgt
501   cgactttagc aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	540	521	2.43e-08

Match details