JRC GMO-Amplicons | EURL GMFF

Record ID:	MT422349.1
Database:	nt	Go to original record
Description:	Veronica nakaiana voucher Y.-E. Lee 2020-001 chloroplast, complete genome.
Taxon:	Veronica nakaiana
Original size:	152319 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46671	47243	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ctaaaaaata aaaaaaaaag
151   gttcagaaag tgaaaaaaaa aggataggtg cagagactcg atggaagctg
201   ttctaacaaa tggagttgac tgtgtcggta gatgaatctt ttcatcgaaa
251   cttcagaaag gatgaaggat aaagggatct attgaatact atataaaaat
301   aaaaaattaa tggtgggccg agtctgtatc tgtatttttt ttagatgaaa
351   aatacaagac ttggtgtaaa ttgattccac attcaagaaa gaatggaata
401   ttcatcaaat cacccactcc atagtctgat agatctttta aagaactgat
451   taatcgggcg agaataaaga tagagtccca ttctacatgt caataccgac
501   aaaaatgaaa tttatagtaa gaggaaaatc cgtcgaattt aaaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details