JRC GMO-Amplicons | EURL GMFF

Record ID:	MT156368.1
Database:	nt	Go to original record
Description:	Salvia pansamalensis chloroplast, complete genome.
Taxon:	Salvia pansamalensis
Original size:	150988 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46370	46933	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaacaaa acaaaggttc
151   aaaaaacgaa aaacaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ccggtagagg aatttttcca cggaaacttt
251   agaaaggatg aaggataaac gcacctattg aatactatat caaatgatta
301   ataatgacta atgttgaccc caatctgttt tttaatatga aaatggaaaa
351   atcgatgtga atttatttcc cgttgaagaa taaatcgaat attcatcaac
401   tcattcactc catagttcga tagatctttt aaagaactta ttaatcggac
451   gagaataaag atagagtccc attctacatg tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details