JRC GMO-Amplicons | EURL GMFF

Record ID:	MT806754.1
Database:	nt	Go to original record
Description:	Fritillaria taipaiensis isolate GZDQ11-2 chloroplast, complete genome.
Taxon:	Fritillaria taipaiensis
Original size:	151692 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44698	45304	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc ttgattttga gaaaaagggt ttaatttaga
151   gtttttataa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   atccttctat caaaattacg gagaggatgc ccgccctata tacgtatata
301   tacatactga catagtaaac gattgattaa tcgcggcccg aatccattat
351   aatatatata tatgaaaaat ttaagagtta ttgtaaatct attccatatt
401   ccaatcaaag tttaaggaag aatcgaatat tcagtgatca aattattcat
451   tccagagtct gtatagatct ttttaaaaac tgattattcg gacgagaata
501   aagagagagt cccattctac gtgtcaatac cgacaacaat gaaatttata
551   gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt caagtccctc
601   tatcccc

View other 14 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details