JRC GMO-Amplicons | EURL GMFF

Record ID:	MT679706.1
Database:	nt	Go to original record
Description:	Cynodon dactylon chloroplast, complete genome.
Taxon:	Cynodon dactylon
Original size:	134072 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46813	47433	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   cagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtgtaatta cgttgtgttg gtagtgaatc tccctctaaa
251   ttactaaatt agagaaagaa gggctttata catctaatac acacgtatag
301   atactgacat agcaaacgat taatcacaga acccatacca taatataggt
351   tctttattta ttttttagaa tgaaattagg aatgattatg aaatagaaaa
401   ttctgaattt tttagaatta ttgtgaatct attccactcg aatattgagt
451   aatcaaaccc ttcaattcat tgtttttgag atcttttaaa aataggatta
501   atcggacgag gataaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details