JRC GMO-Amplicons | EURL GMFF

Record ID:	MT679705.1
Database:	nt	Go to original record
Description:	Panicum maximum chloroplast, complete genome.
Taxon:	Megathyrsus maximus
Original size:	138186 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47075	47687	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctcttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   tatagaaaaa agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccgtatc ataatatagg ttctttattt
351   tattttttag aatgaaattt ggaatgatta tgaaatagaa aattctgaat
401   tttttgagaa ttattgtgaa tccattccaa tcgaatattg agtaatccaa
451   tccttcaact cattgttttc agatctttaa aaaagtggat taatcgaacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details