JRC GMO-Amplicons | EURL GMFF

Record ID:	MT094446.1
Database:	nt	Go to original record
Description:	Schmidtia pappophoroides plastid, complete genome.
Taxon:	Schmidtia pappophoroides
Original size:	133953 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46704	47324	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaaacaag cggttctcaa
151   actagaaccc aaatgaaaag gataggtgca gagactcaat ggaagctatt
201   ctaacgaatc gaggtgtaat tacgttgtgt tggtagtgaa actccctcta
251   aattagagaa agaagggctt tatacatcta atacacacgt atagatactg
301   acatagcaaa cgattaatca cagaacccat atcataatat atataggttc
351   tttatttatt ttttaaaatg aaattaggaa tgattatgaa atagaaaatt
401   ctgaattttt attagaatta ttgggaatcc attccactcg aatattgagt
451   aatcaaatcc ttcaattcat tgtttttgag atcttttaaa aagaggatta
501   atcggacgag gataaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details