JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784681.1
Database:	nt	Go to original record
Description:	Stryphnodendron polystachyum voucher D. Sabatier et al. 3758 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Stryphnodendron polystachyum
Original size:	1087 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	609	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga tttcgtttcg
251   ttagtaaaga gatccttcca tcgaaactcc agaaaagaaa ggatcaagga
301   tgagcatacg tatacgtact aaatactatt tcaattgatt agaccagaca
351   gacccaaaat ctctattttt taatatttat atgacaaata aaagatgtga
401   atcgattcta agttgaagaa agaatcgaat attaattgat caaattcact
451   ccatgatagt ctgatagatt tttggaagaa ctgattaatc agacgagaat
501   aaagatagag tcccattcta catgtcaata ccgacaacaa tgaaatttat
551   agtaagagga aaatccgtcg actgtagaaa tcgttagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details