JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784678.1
Database:	nt	Go to original record
Description:	Stryphnodendron adstringens voucher B.A.S. Pereira et al. 2943 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Stryphnodendron adstringens
Original size:	1064 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	590	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaatcaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga gatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgagcatacg tatacgtact
301   gaaatactat ttcaattgat tagaccagac agaccaaaaa tctctatttt
351   ttaatattta tatgacaaat aaaagatgtg aatcgattct aagttgaaga
401   aagaatcgaa tattaattga tcaaattcac tccatgatag tctgatagat
451   ttttggaaga actgattaat cagacgagaa taaagataga gtcccattct
501   acatgtcaat accgacaaca atgaaattta tagtaagagg aaaatccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details