JRC GMO-Amplicons | EURL GMFF

Record ID:	MW719073.1
Database:	nt	Go to original record
Description:	Hoya diversifolia chloroplast, partial genome.
Taxon:	Hoya diversifolia
Original size:	176087 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	52847	53400	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttgagaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatcct tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atatcaaata attaatgccg acccaaatga
301   gtctgctttt tttctataaa aacggaagaa ttggtgtgat tcgattcttt
351   cttcaatgtg gaatcgaata ttcattgatc aaaagattca ctccatagtc
401   tgtatgtaga tcctcttttc aagaactgga ttaatcggac gagaataaag
451   atagagtccc gttctacatg tcaatgctgg caacaatgca attttgagta
501   agaggaaaat ccgtcgactt aaaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details