JRC GMO-Amplicons | EURL GMFF

Record ID:	MW719056.1
Database:	nt	Go to original record
Description:	Jasminanthes maingayi chloroplast, complete genome.
Taxon:	Jasminanthes maingayi
Original size:	161660 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	53173	53722	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttcagaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatctt tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atatcaaatg attaatgccg accccaatga
301   gtctgtcttt tttctataaa aacggaagaa ttggtgtgat tcgattcttt
351   cttcaatgtg gaatcgaata ttcattgatc aaaagattca ctccatagtc
401   tgtagatcct cttttcaaga actggattaa tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa tgctggcaac aatgcaattt tgagtaagag
501   gaaaatccgt cgacttaaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details