JRC GMO-Amplicons | EURL GMFF

Record ID:	MW719053.1
Database:	nt	Go to original record
Description:	Hoya monetteae chloroplast, complete genome.
Taxon:	Hoya monetteae
Original size:	176921 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	53777	54332	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatt ctgagccaaa tcctattttc aggttgagaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatctt tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atatcaaata attaatgccg acccaaatga
301   gtctgtattt tttctataaa aacggaagaa ttggtgtgat tcgattcttt
351   cttcaatgtg gaatcgaata ttcattgatc aaaagattca ctccatagtc
401   tgtagatcct cttttcaatt tcaagaactg gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatgct ggcaacaatg caattttgag
501   taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details