JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784670.1
Database:	nt	Go to original record
Description:	Piptadenia peruviana voucher M. Nee 38898 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Piptadenia peruviana
Original size:	1090 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	616	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat ataaaaagga taggtccaga gactcaacgg aagttgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga tttcgtttcg
251   ttagtaaaga gatccttcca tcgaaactcc agaaaagaaa ggatcgagga
301   tgagcatacg tatacgtatt gaaatactat ttcaattgat tagaccagac
351   agacccaaaa tctctatttt ttaatattta tatttatatg acaaataaaa
401   gatgtgaatc gattccaagt tgaagaaaga atcgaatatt aattgatcaa
451   attcactcca tgatagtcta atagattttt ggaagaactg attaatcaga
501   cgagaataaa gatagagtcc cattctacat gtcaataccg acaacaatga
551   aatttatagt aagaggaaaa tccgtcgact ttagaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details