JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784668.1
Database:	nt	Go to original record
Description:	Piptadenia paniculata voucher J.G. Jomar et al. 1221 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Piptadenia paniculata
Original size:	1070 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	609	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg acttaattgg attgagcctt ggtatggaaa
51    cctaccaagt gataactttc aaattcagag aaaccctgga attaacaatg
101   ggcaatcctg agccaaatcc tgttttccga aaaccaagaa aagttcagaa
151   aggaagaata taaaaaggat aggtccagag actcaacgga agttgttcta
201   acaaatggag ttgacgacat ttcgtttcgt tagtaaagat ttcgtttcgt
251   tagtaaagag atccttccat cgaaactcca gaaaagaaag gatcgaggat
301   gagcatacgt atacgtattg aaatactatt tcaattgatt agaccagaca
351   gactcaaaat ctctattttt taatatttat atgacaaata aaagatgtga
401   atcgattcaa agttgaagaa agaatcaaat attaattcat caaattcact
451   ccatgatagt ctaatagatt tttggaagaa ctgattaatc agacgagaat
501   aaagatagag tcccattcta catgtcaata ccgacaacaa tgaaatttat
551   agtaagagga aaatccgtcg actttagaaa tcgtgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details