JRC GMO-Amplicons | EURL GMFF

Record ID:	MW770460.1
Database:	nt	Go to original record
Description:	Senecio asperifolius chloroplast, complete genome.
Taxon:	Senecio asperifolius
Original size:	150047 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46835	47357	+	523 bp

Amplicon:

523 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcacgtttt ccgaaaacaa acaaagattc
151   agaaagcgaa aatcaaaaag gataggtgca gagactcgat ggaagttgtt
201   ctaacgaatg gagttgattg tcttacattg gtagaggaat ccttctatcg
251   aaacttcaga aaagatgaag gataaacctg tatacataat acagaagaat
301   tggtatgaat cgattccata ttgaagaaag aatcgaatat ccattgatca
351   aagatcaaac cattcactcc ataatctgat agatcttttg aagaactgat
401   taatcggatg agaataaaga tagagtcccg ttctacatgt caataccggc
451   aacaatgaaa tttatagtaa gaggaaaatc cgtcgatttc aaaaatcgtg
501   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	523	504	2.35e-08

Match details