JRC GMO-Amplicons | EURL GMFF

Record ID:	MW770458.1
Database:	nt	Go to original record
Description:	Phlomoides szechuanensis chloroplast, complete genome.
Taxon:	Phlomoides szechuanensis
Original size:	151872 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46886	47445	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaaac
151   aaaggttcaa aaaacgaact aaaggatagg tgcagagact caatggaagc
201   tgttctaaca aatggagttg actgcgttgg tagaggaatc ctttctacgg
251   aaactttaga aaggataaac gtatctatcg aatactatat aaaatgatta
301   atgatggccc gaatccgtat ttttaatatg aaaaatagaa gaattggtgt
351   gaatttattc tatattgaag aaaaaatcga atattcattc atcaaatcat
401   tcactccata gtccgataga tcttttaaag aactgattaa tcggacgaga
451   ataaagatag agtcccattc tacatgtcaa tactggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details