JRC GMO-Amplicons | EURL GMFF

Record ID:	MW770447.1
Database:	nt	Go to original record
Description:	Alstonia mairei chloroplast, complete genome.
Taxon:	Alstonia mairei
Original size:	178195 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47961	48526	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgatcacttt caaattcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ctatcgaaaa ttcagaaagg
251   atgaaggata aacgtatata cagacgtatt gaatactata tcaaaggatt
301   aatgccgacc cgaatgaatc tgtatttctt ctataagaag aattggtgtg
351   attcgattcc acatggaaga aagaatcgaa tattcattga tcaaatgatt
401   cactccaaag tctgtagatc ttttcaagaa cggattaact gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatgct ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details