JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784664.1
Database:	nt	Go to original record
Description:	Piptadenia irwinii voucher L.P. de Queiroz 3500 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Piptadenia irwinii
Original size:	952 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	620	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaagaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat ataaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga tttcgtttcg
251   ttagtaaaga gatccttcca tcgaaactcc agaaaagaaa ggatcgagga
301   tgagcatacg tatacgtatt gaaatactat ttcaattgat tagaccagac
351   agacccaaaa tctctatttt ttaatattta tatgacaaat aaaagatgtg
401   aatcgattcc aagttgaagc aagttgaaga aagaatcgaa tattaattga
451   tcaaattcac tccatgatag tctgatagat tttttgaaga actgattaat
501   cagacgagaa taaagataga gtcccattct acatgtcaat accgacaaca
551   atgaaattta tagtaagagg aaaatccgtc gactttagaa atcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details