JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ239203.1
Database:	nt	Go to original record
Description:	Stylidium debile chloroplast, complete genome.
Taxon:	Stylidium debile
Original size:	150105 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45684	46269	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcccatttt ccgaaaacaa gagttcaaaa
151   aacaaaaata aagaaggata ggtgcagaga ctcgacggaa gctgttctaa
201   cgaagggagt tgattgtctt gcgttggtat agaatacttt tttgaaaagt
251   ccgaaaagat gaaggataaa cccataatat atatgagtat gtgtacggaa
301   atagtatatc aaaggattcg acgcgagttt tgatcggccc cttttagata
351   aaaaggggaa gaattgttgt aaatcaattt tatcattgaa gaatgaatct
401   actattcatt gatcaaatca ttcacccccg ggtctgataa atcttttgaa
451   ggcctgatta atcggacaag aatagaataa agatagagtc ccattctaca
501   tgtcaatagt ggcaacgatg aaatttatag taagaggaaa atccgtcgat
551   ttttgaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details