JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784657.1
Database:	nt	Go to original record
Description:	Piptadenia buchtienii voucher M. Nee 50497 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Piptadenia buchtienii
Original size:	1084 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	610	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataatttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat ataaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga tttcgtttcg
251   ttagtaaaga gatcctttca tcgaaactct agaaaagaaa ggatcgagga
301   tgagcatacg tatacgtatt gaaatactat ttcaattgat tagaccagac
351   agacccaaaa tctctatttt ttaatattta tatgacaaat aaaagatgtg
401   aatcgattcc aagttgaaga aagaatcgaa tattaattga tcaaattaac
451   tccatgatag tctgatagat ttttggaaga actgattaat cagacgagaa
501   taaagataga gtcccattct acatgtcaat accgacaaca atgaaattta
551   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details