JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784653.1
Database:	nt	Go to original record
Description:	Parapiptadenia zehntneri voucher A.P.S. Gomes et al. 155 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Parapiptadenia zehntneri
Original size:	1094 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	1	2	588	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttaaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga gatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgagcatacg tatacgtact
301   gaaatactat ttcaattgat tagaccagac agacccaaaa tctctatttt
351   ttaatatgga tatgacaaat aaaagatgtg aatcgattct aagttgaaga
401   aagaatcgaa attaattgat caaattcact ccatgatagt ctgatagatt
451   ttggaagaac tgattaatca gacgagaata aagatagagt cccattctac
501   atgtcaatac cgacaacaat gaaatttata gtaagaggaa aatccgtcga
551   ctttagaaat cgtgagggtt caagtccctc ctatccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	95.0	20 bp	0	1	587	568	4.42e-06

Match details