JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ784646.1
Database:	nt	Go to original record
Description:	Mimosa colombiana voucher G. Davidse & O. Huber 15198 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Mimosa colombiana
Original size:	1039 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	595	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagctt tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttca ttagtaaaga gatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgagcgtacg tatacgtact
301   gaaatactat ttccattgat tagaccagac agaccaaaaa tctctatttt
351   ttaatattta tatgaaaaat aaaagatggg aatcgattcc aagttaaaga
401   aagaatcaaa tattctatta attgattaaa ttcactccat gatagtctga
451   tagatttttt gaagaactga ttaatcagac gagaataaag atagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details