Match details

Record ID: EF122473.1
Database: nt Go to original record
Description: Oryza sativa Japonica Group clone KCS315B08 fructose-bisphosphate aldolase mRNA, complete cds.
Taxon: Oryza sativa Japonica Group
Original size: 1494 bp

Method: QT-TAX-ZM-004 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of maize aldolase 1 gene.
Target:maize aldolase 1

View other 211 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 339 407 - 69 bp

Amplicon: 69 bp
1     agggaggaca ccaccctcct tgaggacatc gacgaagggc ttgccgtcct
51    tggtcttctg gtacagggt 

View other 21 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerB 100.0 22 bp 0 0 69 48 2.95e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.