JRC GMO-Amplicons | EURL GMFF

Record ID:	KF539852.1
Database:	nt	Go to original record
Description:	Sisyranthus trichostomus plastid, partial genome.
Taxon:	Sisyranthus trichostomus
Original size:	127337 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48750	49300	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttcggaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatctt tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atagaaaatg attaatgccg accaaaatga
301   gtctgttttt tttctataaa aaacggaaga attggtgtga ttcgattctt
351   tcttcaatgt ggaatcgaat attcattgat caaaagattc actccatagt
401   ctgtagatcc tcttttcaag aaccggatta atcggacgag aataaagata
451   gagtcccgtt ctacatgtca atgctggcaa caatgcaatt ttgagtaaga
501   ggaaaatccg tcgacttaaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details