JRC GMO-Amplicons | EURL GMFF

Record ID:	KF539848.1
Database:	nt	Go to original record
Description:	Eustegia minuta plastid, partial genome.
Taxon:	Eustegia minuta
Original size:	127916 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	53501	54049	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cataaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc acgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaagata tacatacgta ttgaatactc tatcaaatga ttaatgctga
301   cccaaatgag tctgtttttt tttctataaa aaacggaaga attggtgtga
351   ttcgacgtgg aatcgaacat tcattgatca aaagattcac tccatagtct
401   gtagatcctc ttttgaagaa ccggattaat cggacgagaa taaagataga
451   gtcccgttct acatgtcaat actggcaata atgaaatttt gagtaagagg
501   aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details