JRC GMO-Amplicons | EURL GMFF

Record ID:	KF539847.1
Database:	nt	Go to original record
Description:	Astephanus triflorus plastid, partial genome.
Taxon:	Astephanus triflorus
Original size:	133308 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	52472	53031	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcaaaaa ttcagaaagg
251   atgaaagata tacatacgtt ttgaatactt tatcaaatga ttaatgccga
301   cccaaatgag tctgtatttt ttctataaaa aacgtaagaa ttggtgtgat
351   tcgattcttt cttcaatgtg gaattgaata ttcattgatc aaaagattca
401   ctccatagtc tgtagatcct cttttcaaga accggattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgtcaa tactggcaac aatgcaattt
501   ttagtaagag gaaaatccgt cgacttaaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details