JRC GMO-Amplicons | EURL GMFF

Record ID:	MN062350.1
Database:	nt	Go to original record
Description:	Salvia trijuga plastid, complete genome.
Taxon:	Salvia trijuga
Original size:	151345 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46697	47228	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaaaaa aaaaggatag
151   gtgcagagac tcaatggaag ttgttctaac aaatggagtt gactgcgccg
201   gtagaggaat ctttccacgg aaactttaga aaggatgaag gataaaccca
251   tctattgaat actatatcaa atgattaatg ttggcccgaa tctgtttttt
301   taatatgaaa atggaaaaat cgatgtgaat ttatttcacg ttgaagaata
351   aatcgaatat tcatcaactc attcactcca tagttcgata gatcttttaa
401   agaacttatt aatcggacga gaataaagat agagtcccat tctacatgtc
451   aataccggca acaatgaaat ttatagtaag aggaaaatcc gtcgacttaa
501   aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details