JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ274088.1
Database:	nt	Go to original record
Description:	Diospyros mespiliformis voucher BR<BEL>:Meerts & Hasson 509 chloroplast, complete genome.
Taxon:	Diospyros mespiliformis
Original size:	157246 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49421	49982	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgattt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gcgttggtag aggaatcctt ccatcgaaac
251   ctatatacat acgcatacgt actgaaatac tatagcaaac gattaatgac
301   gacccaaatc tgtatccgta ctttttttat atgaaaatgg aagaattgtt
351   gcgaatcgat tccacattga ataaagaatt gactattcat tgatcaaatc
401   atttactcca tagtctgata gatcttttga agaactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aataccgaca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details