JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ274087.1
Database:	nt	Go to original record
Description:	Diospyros crassiflora voucher BR<BEL>:Wursten E5-870 chloroplast, complete genome.
Taxon:	Diospyros crassiflora
Original size:	157874 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49590	50174	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgattt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gcgttggtag aggaatcctt ccatcgaaac
251   tttcgaaagg atgaaagaga aacctatata catacgcata cgtactgaaa
301   tactatagca aacgattaat gacgacccca atctgtatcc gtattttttt
351   tatatgaaaa tggaagaatt gttgcgaatc gattccacat tgaataaaga
401   attgactatt cattgatcaa atcatttact ccatagtctg atagatcttt
451   tgaagaactg attaatcgga cgagaataaa gatagagtcc cattctacat
501   gtcaataccg acaacaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details