JRC GMO-Amplicons | EURL GMFF

Record ID:	MW245830.1
Database:	nt	Go to original record
Description:	Primulina tenuituba chloroplast, complete genome.
Taxon:	Primulina tenuituba
Original size:	153236 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47754	48305	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagcgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ccacgttggt agaagaattg aaacttcaga aaggatgaag
251   gataaacgta tctattgaat actattgatg attaataatg gcccgaatct
301   gtatctgggt attttctata tgaaaaatag aagaattggt gtgaattgat
351   ttcacattga taaaagaatc gaatatttat tagtcaaatc attcactcca
401   cagtccgata gatctcttaa agaacttatt attcggacga gaataaagat
451   agagtcccgt tctacatgtc aataccggca acaatgaaac ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details