JRC GMO-Amplicons | EURL GMFF

Record ID:	MW263073.1
Database:	nt	Go to original record
Description:	Cyclanthera pedata chloroplast, complete genome.
Taxon:	Cyclanthera pedata
Original size:	155030 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	61440	62018	-	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaag gggtaggtgc
151   agagactcaa tggaagctgt tctaacaaat ggagttgact acgttgcgtt
201   agtaaaggaa tgaatccttc tattctatgg aaactccaga aaagatgaaa
251   gataaacgta ttacgtactc aaatactatt tgattaatga caacccgaat
301   ctctattttt ttttataaaa aaaaatgata tataaaaatg aacgaattgt
351   tcggaatcga ttccatacta gttgaaaaaa gaatcgaata ttcattgatc
401   aaatcattta ctccatcata catagtctga tagatttttt gaagaactga
451   ttaatcagat gcgaatagaa taaagataga gtcccattct acatgtcaat
501   accgacaaaa atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details