JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ073339.1
Database:	nt	Go to original record
Description:	Tabernaemontana divaricata chloroplast, complete genome.
Taxon:	Tabernaemontana divaricata
Original size:	157954 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50697	51267	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataat
101   taataaaaag ggcaatcctg agccaaatcc tgttttccac aaacaaaggt
151   tcataaaacg aaaaaaggat aggtgcagag acttaacgga agctgttcta
201   acaaatggag ttggccgcgt tggtagagaa acctttccat cgaaaattca
251   gaaaggatga aggataaacg tatatacata cgtattgaat actatatgaa
301   atgattaatg ccgacccgaa tgaatctgca tttttttcta taaaaaatag
351   aagaattggt gtgattcgat tccacattga agaaagaatc gaatattcat
401   tgatcaaata attcattcca tagtctgtag atcttttcaa gaactgatta
451   atcggacgag aataaagata gagtcccgtt ctacatgtca atgccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgacttaaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details