JRC GMO-Amplicons | EURL GMFF

Record ID:	MW858153.1
Database:	nt	Go to original record
Description:	Clerodendrum cyrtophyllum chloroplast, complete genome.
Taxon:	Clerodendrum cyrtophyllum
Original size:	152004 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47130	47694	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttgctaag tgatgacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttaaaa
151   aacgaaaaaa aaaaggataa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgctggta gaggaatttt tccatggaaa
251   cttcagaaag gatgaaggat aaatggatct attgaatact atatcgaatg
301   attaatgatg gcccgaatct gtatttttta atatgaaaaa tggaagaatt
351   ggtgtgaatt gattccacat tgaagaaaaa atcgaatatt aattcatcaa
401   atcattcact ccacagtccg atagatcttt tcaagatctg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details